Prolonged Survival and Cytoplasmic pH Homeostasis of Helicobacter pylori at pH 1

Prolonged survival and cytoplasmic pH homeostasis of Helicobacter pylori at pH 1.

In the presence of urea, Helicobacter pylori survived for at least 3 h at pH 1. Under these conditions, the cells maintained their cytoplasmic pH at 5.8. De novo protein synthesis during acid shock was not essential for survival of H. pylori at pH 1.

Prolonged Survival and Cytoplasmic pH Homeostasis of Helicobacter pylori at pH

Survival and Chemotactic Behavior of H pylori at Different Media pH

Background: H pylori is a human gastric pathogen. Chemotaxis is an essential factor in colonization of H pylori, but very little is known about its chemotactic responses at different pH conditions, especially in acidic environment of stomach as its natural habitant. Methods: We first determined survival of H pylori under various pH conditions in the presence and absence of urea.  Chemotaxis was...

Expression of the Helicobacter pylori ureI gene is required for acidic pH activation of cytoplasmic urease.

ureI encodes an integral cytoplasmic membrane protein. It is present in the urease gene cluster of Helicobacter pylori and is essential for infection and acid survival, but its role is unknown. To determine the function of UreI protein, we produced H. pylori ureI deletion mutants and measured the pH dependence of urease activity of intact and lysed bacteria and the effect of urea on the membran...

1 CONFORMATIONAL STABILITY OF HELICOBACTER PYLORI FLAVODOXIN . FIT TO FUNCTION AT pH 5

CONFORMATIONAL STABILITY OF HELICOBACTER PYLORI FLAVODOXIN. FIT TO FUNCTION AT pH 5. Nunilo Cremades, Marta Bueno, José Luis Neira, Adrián Velázquez-Campoy, & Javier Sancho Biocomputation and Complex Systems Physics Institute (BIFI), Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza. 50009 Zaragoza, Spain. Instituto de Biología Molecular y ...